Table 2. Kinetic parameters derived from transient optical spectra of Ru–Fd–CoBF2 and Ru–ApoFld–CoBF2 biohybrids in 20 mM Hepes pH 7.9 with 200 mM sodium ascorbate. Samples were excited at 460 nm, and detected across the visible spectrum (Fig. 5 and 7). Kinetic parameters were calculated from global fits of data at 504, 602 and 736 nm (Fig. 6).
| Ru–Fd–CoBF2 | Ru–ApoFld–CoBF2 | |
| τ 1 | 90 ± 20 ns | 170 ± 30 ns |
| τ 2 | 1.5 ± 0.3 μs | 2.5 ± 0.1 μs |
| τ 3 | 6.3 ± 0.2 ms | 810 ± 50 μs |
| τ 4 | 40 ± 4.0 ms | 23 ± 2.9 ms |