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. 2017 Mar 17;7:44902. doi: 10.1038/srep44902

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Copyright © 2017, The Author(s)

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(A) Hemolytic activity of S. pyogenes M6 S165 and its luxR mutant grown in the presence of oxo-C12-HSL. (B) Expression of sagA in S. pyogenes M6 S165 and its luxR mutant grown in the presence of oxo-C12-HSL. (C) EMSA to demonstrate the binding of luxR to the promoter region of sagA. (D) EMSA to demonstrate the bindiding of luxR is specific to DNA from the promoter region of sagA and not affected by AHL. No gel shift for DNA from slo promoter is observed when incubated with oxo-C12-HSL (10 μM). Also, sagA promoter a gel shift was observed even in the absence of oxo-C12-HSL.