Figure 3. Binding of Rex-His₆ to promoter regions of rex, cydA1, atpI, nuoA1, and wblE.

(A) EMSAs using Rex-His₆ protein at the indicated concentrations, and the probes indicated below the panels. Arrow: free probe. (B) ChIP assay analysis of Rex binding to promoter regions of rex, cydA1, atpI, nuoA1, and wblE in vivo. Rex-DNA complexes were immunoprecipitated by anti-Rex antibodies from formaldehyde-treated ATCC31267 and Drex cells. DNAs used for PCR were: total DNA prior to immunoprecipitation (positive control: lanes “+”), immunoprecipitated DNA (experimental sample: lanes “S”), and DNA without antibody (negative control: lanes “−”). hrdB promoter region was used as control.