Table 1. Genotype of the four studied S. cerevisiae strains.
| Strain | Cu importers |
Cu transcription factor | Cu reductases | activity | Genotype | Reference | |
|---|---|---|---|---|---|---|---|
| Ctr1 | Ctr3 | MAC1 | FRE 1 promoter | ||||
 |
(+) | (+) | (+) | wt | +++ | MATα ura3-52 his6 leu2-3,-112 his3-∆200 trp1-901 lys2-801 suc2-∆ | 51 |
 |
(−) | (−) | (+) | wt | +++ | MATα ctr1::ura3::Kanr ctr3::TRP1 his3 lys2-801 CUP1r | 47 |
 |
(−) | (+) | (−) | wt | + | MATα gal1 trp1-1 his3 ade8 CUP1r ctr1::TRP1 mac1 | 47 |
 |
(−) | (+) | (−) | ADH | ++ | MATα gal1 trp1-1 his3 ade8 CUP1r ctr1::TRP1 mac1 pFRE1∆::Kanr-pADH | This study |
(−) knockout, (+) expressed gene.
The relative Cu reductase activity is given by the number of ‘+’ in the 6th column (“activity”). Colors used in this table for each strain are the ones used in the figures.