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. 2017 Mar 17;7:44818. doi: 10.1038/srep44818

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Structure of TAT-NLS-Mad1. (b) P19 cells were grown in suspension in the presence or absence of 30 μM TAT-NLS-Mad1 for a span of 3 days followed by culture in monolayers for 10 days. The cells were immunostained with antibodies against neuronal markers (scale bar: 50 μm). (c) Expression levels of neuronal markers in peptide-treated P19 cells were examined using western blot and (d) RT-PCR analyses. All experiments were conducted at least three times.