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. 2017 Mar 17;7:44775. doi: 10.1038/srep44775

Figure 3. Full-length dystrophin delivery with template-switching vector.

Figure 3

(a) Schematic for the modified dystrophin vector. DNA synthesis must initiate on the 5′ strand and strand transfer to the 3′ LTR will then permit synthesis of a functional provirus following template-switching within the region of dystrophin homology (shaded). A functional provirus can only be produced following recombination because essential elements are split between both strands. (b) Analysis of dystrophin-GFP expression in HEK 293T cells at 4 days post-transduction. Mock-transduced HEK 293T cells were used to set the baseline of GFP fluorescence. Bars represent average GFP readings and standard deviation. All samples are N = 6. **P < 0.01 by Mann-Whitney U test. (c) Nested PCR for full-length dystrophin-GFP from transduced HEK 293T genomic DNA. Products separated on 1% agarose gel show the presence of a 6.2 kb band in sample transduced with the heterozygous vector. L, 10 kb+ ladder; 1, water; 2, mock-transduced HEK 293T; 3, homozygous-transduced HEK 293T; 4, heterozygous-transduced HEK 293T.