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. 2017 Mar 17;7:44705. doi: 10.1038/srep44705

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Copyright © 2017, The Author(s)

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U87 and U251 cells were infected with the empty vector or TUSC3 adenovirus (MOI = 50) for 48 h. (A) The expression of TUSC3 protein was analysed by Western blotting. Full-length blots are presented in Supplementary Figure S7. (B) Cell growth curves for GBM cells were measured by the CCK-8 assay. (C) Transwell assays. (D) Wound-healing assay. (E) Cell cycle progression was analysed by flow cytometry. (F) The cell apoptosis rate was analysed by flow cytometry. The data are expressed as the means ± SD. *P < 0.05, n = 5.