Table 2.
The effects of various metal ions and reagents on the activity of BglP
| Substances | Relative activity (%) | |
|---|---|---|
| 5 mM | 10 mM | |
| Control | 100 | 100 |
| MgCl2 | 101 ± 1.3 | 102 ± 1.2 |
| CaCl2 | 99 ± 0.7 | 96 ± 0.5 |
| MnCl2 | 95 ± 1.6 | 92 ± 1.3 |
| FeCl3 | 98 ± 1.0 | 92 ± 0.9 |
| CoCl2 | 99 ± 0.5 | 86 ± 1.6 |
| ZnCl2 | 98 ± 1.0 | 83 ± 0.4 |
| CuCl2 | 91 ± 1.4 | 44 ± 0.5 |
| FeCl2 | 77 ± 0.8 | 20 ± 0.6 |
| DTT | 99 ± 0.5 | 98 ± 0.9 |
| Urea | 98 ± 1.5 | 96 ± 1.3 |
| EDTA | 96 ± 0.9 | 82 ± 1.4 |
| β-Mercaptoethanol | 99 ± 1.2 | 92 ± 1.6 |
| DMSOa | 5% | 10% |
| 97 ± 0.7 | 90 ± 1.6 | |
| Triton X-100a | 1% | 2% |
| 91 ± 1.0 | 80 ± 0.5 | |
| Tween 80a | 82 ± 0.9 | 73 ± 1.4 |
| SDS | 3.4 ± 0.4 | ND |
The enzymes were pre-incubated for 30 min at 60 °C with each additive before activity measurement with pNPG as substrate. The enzyme activity of BglP without metal ions was taken as 100% (842 ± 15.5 U/mg)
ND not determined. Values are the means ± SD of six experiments (n = 6)
aThe concentrations was percent volume by volume (v/v)