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. 2017 Mar 16;16:119. doi: 10.1186/s12936-017-1765-4

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Identification of Plasmodium species by species-specific nested PCR. A total of 299 blood samples were confirmed to positive by species-specific nested PCR. Representative results are presented. Lane M, 100-bp ladder; lanes 1–4, Plasmodium falciparum (205 bp amplicon); lanes 5–8, Plasmodium vivax (120 bp amplicon); lanes 9–12, mixed with P. falciparum and P. vivax; lanes 13–14, mixed with P. falciparum and Plasmodium malariae (145 bp amplicon); lane 15, mixed with P. vivax and Plasmodium ovale curtisi (800 bp amplicon); lane NC, negative control. No positive case was detected for Plasmodium knowlesi