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. 2017 Mar 17;16:49. doi: 10.1186/s12934-017-0661-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Single clone growth rates in deep-well cultures. Three single clones from YPM (red circles) and BMM-evolved (blue squares) population 1–3 were randomly selected and growth rates were compared to the ancestral P. pastoris strain on YPM (a) and BMM (b). % growth rate relative to the ancestral strain is shown. Number of replicates per single clone, n = 2