Table 1.

Growth rates µ [h⁻¹] of P. pastoris populations in different growth conditions

	YPM	BMM	YPD	YPDN	BMD	BMDN	YPG	BMG
X-33	0.209 ± 0.001	0.089 ± 0.009	0.308 ± 0.002	0.281 ± 0.007	0.312 ± 0.005	0.264 ± 0.007	0.321 ± 0.008	0.269 ± 0.004
X-33 Y250 1	0.227 ± 0.002	0.108 ± 0.001	0.340 ± 0.004	0.278 ± 0.005	0.316 ± 0.007	0.269 ± 0.015	0.353 ± 0.006	0.250 ± 0.003
X-33 Y250 2	0.212 ± 0.006	0.123 ± 0.004	0.372 ± 0.002	0.282 ± 0.005	0.297 ± 0.008	0.205 ± 0.005	0.355 ± 0.007	0.265 ± 0.004
X-33 Y250 3	0.217 ± 0.005	0.142 ± 0.001	0.359 ± 0.005	0.279 ± 0.02	0.302 ± 0.005	0.316 ± 0.014	0.321 ± 0.004	0.285 ± 0.001
X-33 Y250 4	0.216 ± 0.003	0.136 ± 0.005	0.364 ± 0.004	0.291 ± 0.005	0.348 ± 0.011	0.356 ± 0.005	0.354 ± 0.004	0.260 ± 0.002
X-33 M250 1	0.192 ± 0.007	0.129 ± 0.002	0.334 ± 0.002	0.183 ± 0.003	0.286 ± 0.015	0.246 ± 0.017	0.327 ± 0.002	0.257 ± 0.007
X-33 M250 2	0.219 ± 0.003	0.127 ± 0.001	0.328 ± 0.002	0.260 ± 0.032	0.258 ± 0.009	0.240 ± 0.019	0.363 ± 0.002	0.310 ± 0.003
X-33 M250 3	0.199 ± 0.005	0.125 ± 0.007	0.368 ± 0.008	0.264 ± 0.006	0.259 ± 0.010	0.200 ± 0.013	0.350 ± 0.004	0.302 ± 0.011
X-33 M250 4	0.214 ± 0.001	0.126 ± 0.005	0.314 ± 0.03	0.270 ± 0.009	0.256 ± 0.004	0.215 ± 0.005	0.278 ± 0.013	0.289 ± 0.008

Growth tests were performed in 24-deep well plates as described in the “Methods” section. X-33 ancestral strain, X-33 Y250a-d populations evolved on YPM medium, X-33 M250a-d populations evolved on BMM medium, YPM YP medium 1% MeOH, BMM buffered minimal medium 1% MeOH, YPD YP medium 2% glucose, YPDN YPD 500 mM NaCl, BMD buffered minimal medium 2% glucose, BMDN BMD 250 mM NaCl, YPG YP medium 2% glycerol, BMG buffered minimal medium 2% glycerol; values represent averages ± standard error (n = 4). Growth rates in italics differ significantly from X-33 ancestral growth rates (p < 0.05, Additional file 1: Table S3)