Table 2.
The final OD600 of ancestral and evolved populations
| YPM | BMM | YPD | YPDN | BMD | BMDN | YPG | BMG | |
|---|---|---|---|---|---|---|---|---|
| X-33 wt | 2.65 ± 0.09 | 0.83 ± 0.01 | 6.46 ± 0.13 | 4.56 ± 0.22 | 4.07 ± 0.15 | 3.09 ± 0.26 | 6.05 ± 0.16 | 7.98 ± 0.32 |
| X-33 Y250 1 | 2.67 ± 0.07 | 1.26 ± 0.02 | 7.24 ± 0.13 | 4.34 ± 0.15 | 4.20 ± 0.02 | 2.11 ± 0.09 | 4.23 ± 0.10 | 8.77 ± 0.22 |
| X-33 Y250 2 | 2.94 ± 0.07 | 1.05 ± 0.02 | 7.27 ± 0.10 | 4.80 ± 0.07 | 4.78 ± 0.10 | 2.55 ± 0.09 | 4.01 ± 0.20 | 8.02 ± 0.17 |
| X-33 Y250 3 | 2.96 ± 0.02 | 0.81 ± 0.01 | 7.69 ± 0.04 | 4.35 ± 0.04 | 4.33 ± 0.10 | 2.35 ± 0.11 | 4.87 ± 0.13 | 6.84 ± 0.09 |
| X-33 Y250 4 | 3.13 ± 0.04 | 1.01 ± 0.02 | 7.60 ± 0.15 | 4.44 ± 0.05 | 4.31 ± 0.10 | 2.18 ± 0.08 | 4.74 ± 0.05 | 7 47 ± 0.15 |
| X-33 M250 1 | 2.63 ± 0.0.3 | 1.42 ± 0.01 | 7.08 ± 0.20 | 4.57 ± 0.08 | 3.70 ± 0.22 | 4.04 ± 0.02 | 5.52 ± 0.16 | 7 26 ± 0.26 |
| X-33 M250 2 | 2.59 ± 0.08 | 1.43 ± 0.02 | 6.86 ± 0.12 | 4 52 ± 0.04 | 3.53 ± 0.09 | 3 18 ± 0.15 | 5.96 ± 0.13 | 7.14 ± 0.22 |
| X-33 M250 3 | 3.33 ± 0.05 | 1.62 ± 0.01 | 6.79 ± 0.14 | 4 59 ± 0.06 | 4.28 ± 0.12 | 3.52 ± 0.15 | 6.10 ± 0.17 | 6.08 ± 0.07 |
| X-33 M250 4 | 2.61 ± 0.05 | 1.43 ± 0.04 | 7.25 ± 0.05 | 4 27 ± 0.05 | 4.28 ± 0.12 | 3.36 ± 0.17 | 5.19 ± 0.17 | 6.23 ± 0.20 |
Growth tests performed in 24-deep well plates as described in the “Methods” section. X-33 ancestral strain, X-33 Y250a-d populations evolved on YPM medium, X-33 M250a-d populations evolved on BMM medium, YPM YP medium 1% MeOH, BMM buffered minimal medium 1% MeOH, YPD YP medium 2% glucose, YPDN YPD 500 mM NaCl, BMD buffered minimal medium 2% glucose, BMDN BMD 250 mM NaCl, YPG YP medium 2% glycerol, BMG buffered minimal medium 2% glycerol; Measurements were performed after 24 h of cultivation (BMM after 48 h). Values represent averages ± standard error (n = 4)