Fig. 4.

The effect of addition or inhibition of TGF-β and Hsp90 on migration of the paired SW480 and SW620 colon cancer cell lines. SW480 a and SW620 b cells were treated with 2 ng/ml TGF-β1, 20 ng/ml Hsp90β, 100 nM SB431542, 100 μM novobiocin and 10 μg/ml αvβ6 integrin blocking antibody (anti- αvβ6) singly or in combinations as indicated on the x-axis and the effect of such treatment on migration assessed. All graphs are representative of the average of three independent experiments performed in triplicate and error bars indicate the standard error in the mean. Statistical analysis was performed using GraphPad Prism 4.03 software. A two-way analysis of variance (ANOVA) with Bonferroni post-test was performed and significance between untreated cells and those after each treatment (indicated by asterisks) and between particular treatments (indicated by hashes) are shown (*p < 0.05, **p < 0.01, ***p < 0.001, ^## p < 0.01, ^### p < 0.001, ns – not significant)