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. 2017 Mar 17;7:44671. doi: 10.1038/srep44671

Table 2. PA biosynthesis-related genes expression undergoing natural and water-treated de-astringency.

Gene Enzyme Alla 10W-vs-20Wb 10W-vs-10Tc 10W-vs-10Ad
Up Down Up Down Up Down
DAHPS 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase 4 0 0 1 3 0 3
DHQS 3-dehydroquinate synthase 2 0 1 0 2 0 1
DHD 3-dehydroquinate dehydratase 16 0 1 0 0 0 1
SK Shikimate kinase 15 0 2 2 0 0 0
EPSPS 5-enolpyruvylshikimate 3-phosphte synthase 1 0 0 0 1 0 0
CS Chorismate synthase 5 0 2 1 0 0 0
PAL Phenylalanine ammonia-lyase 14 0 0 10 13 0 5
C4H cinnamate-4-hydroxylase 16 0 7 0 9 0 10
4CL 4-coumarate: coenzyme A ligase 23 2 0 1 6 0 4
CHS Chalcone synthase 4 0 3 0 2 0 2
CHI Chalcone isomerase 4 0 2 1 2 0 3
F3H Flavanone 3-hydroxylase 8 0 1 1 3 0 1
F3′H Flavonoid 3′-hydroxylase 6 0 3 0 3 0 3
F3'5H Flavonoid 3′5′-hydroxylase 5 0 1 0 2 0 1
DFR Dihydroflavonol 4-reductase 41 2 1 4 1 2 1
ANS Anthocyanidin synthase 1 0 1 0 1 0 1
LAR Leucoanthocyanidin reductase 1 1 0 0 0 0 0
ANR Anthocyanidin reductase 13 0 4 3 3 0 4
GST Glutathione S-transferase 85 3 0 20 2 0 0
MATE Multi-drug and toxic compound extrusion transporter 44 4 2 1 6 0 5
LAC Laccase 13 1 2 4 4 0 2

aAll, the total number of analysed uni-transcripts. b10W-vs-20W, the ratio of genes expressed in ‘Eshi 1′ fruit at 20 WAB compared to those at 10 WAB. c10W-vs-10T, the ratio of genes expressed in ‘Eshi 1′ fruit sampled at 10 WAB and then treated with 40 °C water for 36 h compared to those in untreated fruits. d10W-vs-10A, the ratio of genes expressed in ‘Eshi 1′ fruit sampled at 10 WAB and then treated with 25 °C water for 12 h compared to those in untreated fruits.