. Author manuscript; available in PMC: 2017 Mar 17.

Published in final edited form as: Biochemistry. 2016 Aug 23;55(35):4971–4981. doi: 10.1021/acs.biochem.6b00347

Table 1.

List of ApoA-I Molecules Used in This Study

apoA-I helical domain ^{amino acid}		formation of intramolecular disulfide		formation of rHDL particles			mass of mutant apoA-I from mass spectrometry/disulfide ionⁿ⁺

		predicted	actual	apoA-I modification	size (nm)	POPC/apoA-I ± SD
N^D13C	H2^V67C	no	no	unlocked	9.6	54:1 ± 6	28142
N^D13C	H2^V67C	no	no	alkylated	9.8/9.6	44:1 ± 4	28143
N^G26C	H1^K59C	no	no	unlocked	9.6/8.0	54:1 ± 6	28171
N^G26C	H1^K59C	no	no	alkylated	9.8	56:1 ± 5	28170
N^E34C	H1^S55C	yes	yes	locked	9.8	36:1 ± 5	28140/751.34⁴⁺
N^E34C	H1^S55C	yes	yes	alkylated	9.6	58:1 ± 5	28140
H1^V53C	H5^R123C	yes	yes	locked	NPF^a	^b	28119/758.35⁴⁺
H1^V53C	H5^R123C	yes	yes	alkylated	9.6	53:1 ± 14	28119
H4^D103C	H7^R177C	yes	yes	locked	9.4	^b	28137/733.04³⁺
H4^D103C	H7^R177C	yes	yes	alkylated	9.6	56:1 ± 10	28137
H4^F104C	H6^M148C	no	no	unlocked	9.6	69:1 ± 1	28078
H4^F104C	H6^M148C	no	no	alkylated	9.6	50:1 ± 4	28078
H4^F104C	H6^R160C	yes	yes	locked	NPF^a	^b	28053/804.88⁴⁺
H4^F104C	H6^R160C	yes	yes	alkylated	9.6	64:1 ± 5	28053
H4^F104C	H6^H162C	yes	yes	locked	NPF^a	28:1 ± 3	27998/820.02³⁺
H4^F104C	H6^H162C	yes	yes	alkylated	9.6	64:1 ± 10	27998
H6^D157C	H7^L178C	yes	yes	unlocked	8.0	^b	28128/665.34²⁺
H6^D157C	H7^L178C	yes	yes	alkylated	9.6	60:1 ± 8	28129
H9^L200C	H10^L233C	yes	yes	locked	9.6	54:1 ± 2	28130/645.55⁴⁺
H9^L200C	H10^L233C	yes	yes	alkylated	9.8	64:1 ± 4	28130
wild-type apoA-I		not applicable		none	9.6	52:1 ± 4	28150

No particle found by nondenaturing gel electrophoresis.

No lipid phosphorus was detected.