Figure 1.
Phylogenetic tree of the H5 gene sequences, three avian influenza H5 viruses, France, November 2015
HA: haemagglutinin; HPAI: highly pathogenic avian influenza.
The complete coding sequences of the three H5 HPAI virus isolated from poultry in France were aligned with their closest related sequences as detected by BLAST, together with other sequences selected as representatives for more distant clusters. Then, the neighbour-joining method based on the Kimura-2 parameter model was applied using the MEGA 6 software [26] to obtain a phylogenetic tree with 1,000 bootstrap replicates. Only bootstrap values higher than 75% are shown. The HA sequences of French viruses are indicated with black dots. The tree was rooted by an H2 sequence.
