Skip to main content
. 2016 Sep 16;7(42):67796–67807. doi: 10.18632/oncotarget.12080

Figure 5. miR-200b targets the 3′UTRs of Eps8, Lhfp, Glis2 and Rps6kb1.

Figure 5

A. TargetScan identified the conserved predicted miR-200b binding sites on the 3′UTRs of Eps8, Lhfp, Glis2 and Rps6kb1. B.-E. Quantification of the dual-luciferase assay in HEK293 cells co-transfected with an miR-200 mimic or control oligonucleotides and the Eps8 B., Lhfp C., Glis2 D., Rps6kb1 E. or mutant 3′UTR reporter plasmid (n = 4). F. mRNA expression of Eps8, Lhfp, Glis2 and Rps6kb1 genes in Gon-fat of the WT (black bars) and ASKO (white bars) mice (n = 6). mut, mutant. The values are expressed as the means ± SEM. *p < 0.05, **p < 0.01.