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Cardiac protein expression of TGF-β, Smad2 phosphorylation, Smad3 phosphorylation, ERK1/2 phosphorylation and p38 phosphorylation. Anti-GAPDH antibody served as a loading control. Values are mean ± SEM. n = 4-8 in each group. *P < 0.05, **P < 0.01.
