Figure 7. Immunization of B cell-deficient mice results in equivalent frequencies of antigen-specific CD8 T cells as measured by tetramer staining, but lower antigen-specific IFNγ secretion by CD4 and CD8 T cells.

Age-matched B cell-deficient BL6 μMT mice and wild-type C57BL6 mice (n = 6/treatment group) were immunized four times at weekly intervals with 100μg of psOVA by intradermal injection. a. One week after the final injection, frequencies of antigen-specific CD8 T cells in the spleen were assayed by SIINFEKL specific tetramer staining. b. CD8 and CD4 T cells were assayed for peptide antigen-specific release of IFNγ by intracellular cytokine staining and flow cytometric analysis. For all panels, * denotes a p-value < 0.05, two-sided t-test. Data are representative of 2 independent experiments. Error bars represent mean + SEM.