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. 2016 Sep 1;7(42):67966–67985. doi: 10.18632/oncotarget.11817

Figure 2. US28 constitutively induces the transcriptional activity of the HIF-1 complex by increasing the HIF-1α protein level.

Figure 2

A. Stably transfected mock and US28 NIH-3T3 cells were used for RNA isolation. Quantitative RT-PCR is performed to determine HIF1A relative mRNA expression levels, using ACTB as normalization control. Lysates were prepared from stably transfected NIH-3T3 cells with US28 or control vector B. or U251-iUS28 cells with inducible US28 expression C., expression of HIF-1α protein was evaluated with a specific antibody by Western blotting, followed by signal quantification using β-actin expression as a loading control. Significant differences between conditions are depicted by asterisks (** = P < 0.01, *** = P < 0.001)