Table 1. Overview of US28 HIF-1 reportergene data.
| Inhibitor | Target | Reporter only inhibition (%) | US28 inhibition (%) | US28-selective inhibition (p value) |
|---|---|---|---|---|
| Pertussis Toxin | Gαi | −5 | −5 | 0,886 |
| UBO-QIC | Gαq | 1 | 71 | 1,30E-06 |
| Gallein | Gβγ | 11 | 2 | 0,057 |
| U73122 | Phospholipase C | 2 | 2 | 0,928 |
| KN-93 | CaMKII | 30 | 65 | 1,20E-04 |
| LY-294002 | PI3K | 29 | 18 | 0,145 |
| Rapamycin | mTOR | 71 | 76 | 0,024 |
| GSK690693 | Akt | 56 | 50 | 0,084 |
| BAY 11-7082 | NF-κB | 9 | −1 | 0,581 |
| H89 | PKA/ROCK | 30 | 43 | 0,027 |
| Y27632 | ROCK | 2 | −7 | 0,224 |
| P6 | JAK | 0 | −3 | 0,640 |
| Stattic | STAT3 | 11 | −11 | 0,021 |
| Bisindolylmaleimide | PKC | 68 | 68 | 0,862 |
The applied inhibitors and their pharmacological targets, inhibition of transcriptional activity in HIF-1 reporter only transfected cells and inhibition on US28 and HIF-1 reporter transfected cells. Multiple t-tests with Holm-Sidak correction were performed to determine statistically significant (α=0.05) US28-selective inhibition (P value).