Figure 3. SJ26 repressed WNT1 expression in a G-quadruplex dependent manner.

A. Suppression of WNT1 expression by SJ26 using reporter-harboring stable cell lines. The H1299 cells harboring wild-type, WNT1-m1, and WNT1-m6 reporters were incubated with the indicated concentrations of SJ26 for 24 h. The phosphatase activities were then analyzed using the activity of DMSO-treated cells as 100%. B. Cytotoxic effects of SJ26. About 2 × 10⁴ H1299 cells were seeded in 96-well plates and incubated with SJ26 at various concentrations for 24 h. Cell growth was then determined using the MTT assay. The values are obtained from three experiments using the values of untreated cells as 100%. C. Suppression of WNT1 expression by SJ26 using transient-transfected reporter assays. Reporter plasmid constructs carrying wild-type, WNT1-m1, and WNT1-m6 promoters were transfected into H1299 cells using lipofecamin 2000. The transfected cells were incubated with the indicated concentrations of SJ26 for 24 h. The phosphatase activities were then analyzed using the activity of DMSO-treated cells as 100%. Asterisks indicate p < 0.05.