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. 2016 Jul 29;7(42):68057–68071. doi: 10.18632/oncotarget.10929

Figure 6. Tube formation assay.

Figure 6

HUVEC were pre-treated or not with a blocking anti-NRP1 antibody (ab) treated with miRNA mimics, and plated onto Matrigel. The images were taken after 2, 6, and 20 h. The tube formation was quantified as the total length of tubules using ImageJ. A. Tube formation by HUVEC treated with miR-20a for 20 h. B. Calculated tube length formed by the cells treated with miRNA mimics. The data is presented as Mean±SEM, and statistical significance is denoted as: * P<0.05, ** P<0.01, **** P<0.0001. The data is representative of three independent experiments.