Table 1. Binding affinity of miRNAs to sNRP1 and NRP1-Fc and the effect of cations.

miRNAxs	Immobilized protein	Kd, nM (no Mg, no Ca2+)	Kd, nM (0.5 mM Mg, no Ca2+)	Kd, nM (1.5 mM Mg, no Ca2+)	Kd, nM (at 0.9 mM Mg+1.2 mM Ca2+)
miR-20a	sNRP1		0.02823±0.00975		0.3864±0.02742
miR-138	sNRP1		0.3484±0.0814		0.7720±0.07681
miR-331	sNRP1	1.2±0.7	0.03830±0.01483	Kd=0.071±0.025	0.8500±0.1688
miR-331	NRP1-Fc				0.9112±0.3860
miR-422	sNRP1		0.1375±.02458		0.3441±0.0657

*The data is representative of 6 independent experiments.