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. 2016 Sep 10;7(42):68292–68302. doi: 10.18632/oncotarget.11944

Figure 5. Cyclic stretch-induced expression of IL-1β and pyroptosis were caspase-1 dependent.

Figure 5

(A) The addition of the caspase-1 inhibitor significantly inhibited the appearance of IL-1β in the culture medium of the cyclic stretched cells, compared with the non-inhibited 6 h stretched cells. (B) The pyroptotic rate of HPDLCs in response to 6 h cyclic stretch increased significantly. While the addition of the caspase-1 inhibitor significantly inhibited the pyroptotic rate in response to 6 h cyclic stretch, compared with the non-inhibited 6 h stretched cells. (C) Western blot analysis showed that 6 h cyclic stretch increased the expressions of pro-IL-1β and mature IL-1β proteins. While the addition of the caspase-1 inhibitor significantly inhibited the expressions of both pro-IL-1β and mature IL-1β, compared with the non-inhibited 6 h stretched cells. The GAPDH was used as a loading control. results were quantified from at least 3 independent experiments and expressed as mean ± S.E. Statistical significance was calculated using one-way ANOVA with multiple comparisons. *P < 0.05 versus control group; #P < 0.05 versus 6 h group.