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. 2016 Aug 31;7(42):68385–68396. doi: 10.18632/oncotarget.11724

Figure 5. CATS knockdown reduced U937 cell migration towards serum containing media.

Figure 5

Migration was evaluated by counting the number of cells that passed through the 8μm pore-sized transwell plates and were present in the lower compartment after 24h exposure to the chemotatic stimuli. A. Migration towards 0.5% BSA (used as a control), 10% FBS, CXCL12 and 10% FBS + CXCL12 containing media. The bars represent the number of migrating cells normalized by the input, expressed as percentage. Results are shown as mean ± SD of at least three independent duplicate experiments. B. Western blot analysis of shControl and shCATS total cell extracts. Membrane was blotted against anti-Ac-α-TUBULIN, anti-α-TUBULIN, anti-SIVA and anti-ACTIN, used as a control for equal sample loading (upper panel) and against anti-STMN1 (Ser16), anti-STMN1 (Ser38), anti-STMN1 and anti-ACTIN, used as a control for equal sample loading (lower panel). The bar graphs represent densitometric analysis of blots relative to control set as 100% (UN-SCAN-IT software). *p<0.05, Student's t test.