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. 2016 Aug 31;7(42):68385–68396. doi: 10.18632/oncotarget.11724

Figure 6. CATS knockdown reduces colony formation and GLI-1 mRNA expression in U937 cells.

Figure 6

A. Colony formation assay. Colonies containing viable cells were stained with MTT after 8 days of culture. Images of representative culturing plates are shown. The bars represent the number of colonies formed normalized by the control and expressed as percentage. Results are shown as mean ± SD of six independent triplicates experiments. B. Relative expression of the self-renewal regulators (NANOG, BMI-1 and GLI-1) and the CALM/AF10-leukemia deregulated genes (HOXA5, HOXA7, HOXA9, HOXA10, HOXA11 and MEIS1) in shCATS and shControl cells. Results are shown as mean ± SD of at least three independent triplicates experiments. Expression levels of mRNA were normalized by HPRT. *p<0.05; **p<0.01, Student's t test.