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. 2016 Aug 31;7(42):68397–68411. doi: 10.18632/oncotarget.11722

Figure 5. miR-140-5p directly targets IGF2BP1 in CC cells.

Figure 5

A. Targetscan program predicts that IGF2BP1 is a direct target gene of miR-140-5p. B. The wild-type (WT) or mutant (MUT) binding sequences of miR-140-5p on IGF2BP1 3′-UTR. C. qRT-PCR analysis of IGF2BP1 mRNA levels in 21 CC tissues and 8 Normal cervix tissues. D. Spearman's correlation analysis between miR140-5p levels and IGF2BP1 mRNA levels in 21 CC tissues. E. qRT-PCR analysis of the effects of miR-140-5p over-expression and inhibitor on IGF2BP1 mRNA levels in C33A and HeLa cells. F. IHC analysis of IGF2BP1 protein levels in normal cervix tissues and CC tissues. G. 3′UTR luciferase assay analysis of the capacity of miR-140-5p binding to the 3′UTR of IGF2BP1. H. Western blot analysis of the effects of miR-140-5p over-expression and inhibition on IGF2BP1 protein levels in C33A and HeLa cells.