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. 2016 Sep 2;7(42):68546–68558. doi: 10.18632/oncotarget.11843

Figure 1. Inhibition of PI3K p110α decreases viability in HCT116 and SW480 CRC cells.

Figure 1

The effect of siRNA-mediated targeted depletion of MEK1/2 and PIK3CA on cellular viability was evaluated in HCT116 A. and SW480 B. CRC cells. Briefly, cells were grown, transfected with siRNA for MEK1 and MEK2 or PIK3CA and cellular viability determined by MTS assay 72h after transfection. Representative effects of MEK1/2 or PI3K p110α downregulation on HCT116 cells are shown (C, original magnification: x100). Representative Western blot D, F. and corresponding quantification analysis E, G. of MEK1/2, PI3K p110α (D, E) and PI3K p110β (F, G) upon silencing of MEK1 and MEK2 or PIK3CA in HCT116 CRC cells. Briefly, cells were grown, transfected with siRNA for MEK1 and MEK2 or PIK3CA and protein extracted 72h after transfection. Protein levels were assessed by Western blot analysis and subsequent quantification was performed. Controls included cells transfected with transfection reagent (mock ctrl) and Non-targeting siRNA. Data represent means ± SEM of at least triplicate experiments normalized to controls. All conditions were compared with Non-targeting siRNA. Ctrl, control. **, p< 0.01;****, p< 0.0001.