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. 2016 Sep 6;7(42):68623–68637. doi: 10.18632/oncotarget.11864

Figure 2. Effects of DCTPP1 knockdown on 5-FU-induced apoptosis in BGC-823 cells.

Figure 2

A. Cells were treated with or without 100 μM 5-FU for 48 h and apoptosis was examined by using FITC-Annexin V/PI staining. The fluorescence intensity of FITC-Annexin V was plotted on the x-axis, and PI was plotted on the y-axis. FITC−/PI−, FITC+/PI−, FITC+/PI+, FITC−/PI+ was regarded as living, early apoptotic, late apoptotic and necrotic cells, respectively. B. The statistical analysis of apoptotic BGC-823 cells (FITC+) with or without 5-FU treatment. C. Caspase-3 and cleavage caspase-3 levels in DCTPP1-knockdown and control BGC-823 cells upon 5-FU treatment were measured by Western blot. All the values shown were represented as means ± SD. (ns: not significant; ***: P < 0.001 vs control by two-tailed Student's t-test).