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. 2016 Sep 8;7(42):68749–68767. doi: 10.18632/oncotarget.11899

Figure 8. Glucose and Lactate measurement, Oxygen consumption, and mitochondrial complexes activity in HT29 cells.

Figure 8

A and B. 1 × 106 cells were stimulated with PDGF (100 ng/ml) for 24 hours. Unstimulated cells and cells treated with PDGF were washed with glucose-free buffer (110mM NaCl, 5mM KCl, 1mM MgCl2, 4mM Na2PO4, 50mM Na-HEPES, pH 7.4), and treated with Glucose (20mM), GLUT1 inhibitor WZB117 (10 μM) or both. Glucose uptake (A), and lactate release (B) was measured with an abcam Glucose and Lactate Assay. HT29 cells were treated with PDGF or VEGF or both PDGF and VEGF (100 ng/ml) for 24 hours, n=3. Oxygen consumption C. showed no changes during PDGF and/or VEGF stimulation. For mitochondrial activity measurement D-H., cells were treated with PDGF, VEGF or both for 30 minutes, and measured with a MILLIPLEX® human oxidative phosphorylation (OXPHOS) magnetic bead panel, n=2. PDGF and VEGF decreased the activity of the mitochondrial complexes.