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. 2016 Sep 8;7(42):68749–68767. doi: 10.18632/oncotarget.11899

Figure 9. Investigation of HIF1α in human colon cancer probes (A), and in HT29 cells (B) and c-Myc (C and D) in HT29 cells (E).

Figure 9

Gene expression of HIF1α was increased on gene level at stage UICC III/IV in the human colon cancer probes A. and during PDGF- and VEGF-stimulation (100 ng/ml) in HT29 cells B. C and D. Lower panels show representative western blots and upper panels show quantification of three independent western blot experiments of pc-Myc (S62), normalized to β-Actin loading control. Pc-Myc activity was increased by PDGF, and decreased by Akt inhibitor. Cells were treated with PDGF (100 ng/ml), Akt inhibitor IV (10 μM) (C) or PI3K Inhibitor (80 nM) (D). Results are presented as ±SD, *p<0.05, **p<0.01, ***p<0.001.