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. 2016 Sep 15;7(42):68864–68882. doi: 10.18632/oncotarget.12025

Figure 3. Cy-3-glu induces TNBC MDA-MB-231 cell death by the extrinsic apoptosis pathway, not necroptosis or by the intrinsic pathway.

Figure 3

MCF-7 and MDA-MB-231 cells were treated with Cy-3-glu (150 μM) for 24 h in FBS-free cell culture medium. A. left panel, bar graphs show the relative protein levels of RIP3. Right panel, RIP3 expression in MDA-MB-231 detected by Western blotting; the mouse pancreas was used as the positive control. B. upper panel, expression levels of cleaved-caspase-3 (17 kDa and 19 kDa), cleaved-caspase-8 (47 kDa), caspase-3 (35 kDa) and caspase-8 (57 kDa). β-actin was the internal control. Lower panel shows the relative protein levels of cleaved-caspase-8 and cleaved-caspase-3 in MCF-7 and MDA-MB-231 cells. The results represent the mean ± SEM from three independent experiments. Differences with p < 0.05 (*), p < 0.01 (**) or p < 0.001(***) are considered statistically significant.