Table 2. The sequences of the oligonucleotide primers and amplified products of real-time PCR.
| Gene name | Sequence of primer (5′–3′) | Amplified product (bp) |
|---|---|---|
| GAPDH | F: ATGGGGAAGGTGAAGGTCG | 107 |
| R: GGGGTCATTGATGGCAACAATA | ||
| Cyclin D1 | F: ACCTGAGGAGCCCCAACAA | 105 |
| R: TCTGCTCCTGGCAGGCC | ||
| Cyclin B1 | F: TGGACTATGACATGGTGCACTT | 156 |
| R: GCCAGGTGCTGCATAACTG | ||
| Cyclin E | F: CGCCTGCCGGGACTGGAG | 110 |
| R: TCTTCCTGGAGCGAGCCG | ||
| CDK2 | F: CCCTTTCTTCCAGGATGTGA | 134 |
| R: TGAGTCCAAATAGCCCAAGG | ||
| CDK4 | F: GAAACTCTGAAGCCGACCAG | 78 |
| R: ACATCTCGAGGCCAGTCATC | ||
| CDK6 | F: TCCCTCCTTTGAAGTGGATG | 148 |
| R: GTCACCTGGGGCTAAATGAA | ||
| EGFR | F: AGGCACGAGTAACAAGCTCAC | 176 |
| R: ATGAGGACATAACCAGCCACC |
F: forward; R: reverse; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; CDK2: cyclin-dependent kinase 2; CDK4: cyclin-dependent kinase 4; CDK6: cyclin-dependent kinase 6; EGFR: epidermal growth factor receptor.