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NFI and Oct-1 bind the Ad5 origin of replication simultaneously. Binding of NFI and Oct-1 to the Ad5 origin was studied by band shift assays using the TD50 probe containing the first 50 nt of the Ad5 origin. In lanes 1–4 and 5–8, respectively, 0.17 pmol and 35 fmol of TD50 probe were used. The position of the protein–DNA complexes and free probe are marked with arrowheads. Lanes 1 and 5 represent free probe.
