Figure 1. Primary cells from B cell lymphoma patients are resistant to conventional chemotherapeutic agents and contain significantly lower cytosolic Apaf-1.
a., b. 2×105 primary lymphocytes/200μl/well in 96 well plates were exposed to Etoposide (5μM), Daunorubicin (0.4μg/ml), Vincristine (5μM), MPO (2.5μM), MPO-Zn (200nM), C1 (50μg/ml), LY30 (50μM) or TRAIL (100ng/ml) for 24 hrs. Cell viability was determined by the MTT assay. One way ANOVA multiple comparisons analysis was used for statistical significance (* p < 0.1, **p < 0.001, #p < 0.5, ****p < 0.0001) c. S-100 cytosolic and pellet fractions of primary cells from B-cell and T-cell lymphomas as well as from Raji and Jurkat cell lines were resolved by SDS-PAGE, transferred to PVDF and Apaf-1 was detected by immunoblotting using anti-Apaf-1. Membranes were probed with anti-β-actin and anti-VDAC-1 as loading controls for cytosolic and pellet fractions, respectively.