Figure 4. IL-4-activated macrophages induce vascular tube formation in U87.

A. U87 cells were plated on matrigel for tube formation. HUVEC served as a positive control. B. Tubules formed by U87 cells were quantified. Total number of tubules in x100 view was comparedamong the three groups. The results revealed that U87 cocultured with M₂ macrophages were much easier to form vascular-like channels compared with the other two groups. C. Western blot showed that coculturing with M₂ macrophages could increase COX-2 and SMA expression in U87 cells, while VE-cadherin expression was bare to detect.