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. 2016 Nov 16;7(51):84054–84071. doi: 10.18632/oncotarget.13414

Figure 3. PI3Kβ regulates UBC cell survival in a kinase-independent manner.

Figure 3

a.-c. 639V cells were cultured with AZD8186 (dose indicated) or were transfected with siPIK3CB at days 1 and 3, and examined at day 5. Images show representative fields a., percentage of surviving cells at experiment termination compared to control cells (100%) b., and WB examination of cell extracts c.; pPKB signal normalized to PKB is indicated at the bottom c.. d., e. 639V cells were transfected with control or siPIK3CB alone or in combination with ER-myc-K805R-p110β (KR-PI3Kβ) or the siPIK3CB-resistant mutant (KR-PI3Kβ-si-res) (72 h). All KR-p110β-transfected cells were treated with tamoxifen (1 µM) for 12 h before analysis. Representative images d. and percentage of surviving cells e. analyzed as in b.. f. WB showing recombinant KR-PI3Kβ (examined with anti-myc Ab), pPKB, and controls; pPKB signal was quantitated as in c.. ** P < 0.01, *P < 0.05; Student's t-test.