Figure 6. E-cadherin depletion sensitizes.

PTENmutant UBC cells for PIK3CB silencing. a. Xenografts were generated by injecting E-cad-depleted UMUC-9 cells (5 x 10⁶) in PBS:Matrigel (1:1) into both flanks of immunodeficient mice. When most tumors reached ~75 mm³, doxycycline was added to drinking water and tumor size measured every 3 days. The graph shows the x-fold increase in tumor size (mean ± SD) at different times relative to the volume at treatment initiation (day 1). *** P < 0.001; ** P < 0.01; one-way ANOVA Tukey's multiple comparison test. (b, c) Representative histochemistry b. and TUNEL analysis c. of indicated tumor sections.