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. 2016 Aug 23;7(51):84128–84141. doi: 10.18632/oncotarget.11554

Figure 2. Lunasin depleted populations of cells displaying high ALDH activity.

Figure 2

A375 and SKMEL-28 cells showed a substantial decrease in ALDH positive populations when treated with Lunasin for 24 h. DEAB was used as a negative control and served as a tool for gating ALDH negative populations; the gating was kept constant for each cell line and is indicated within each plot. Representative flow cytometry plots and corresponding fluorescent microscopy images were taken at 24 h post-treatment and provided for DEAB (A, D), control (B,E), and Lunasin-treated (C, F) groups. Lunasin reduced the number ALDHhigh cells in A375 and SKMEL-28 cell lines when compared to vehicle-treated cells (G). Statistical significance was determined from three independent experiments and assessed by student t-test (p < 0.05). Fluorescent microscopy images were taken at 40× magnification. Error bars represent mean ± S.D.