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. 2016 Oct 4;7(51):84246–84257. doi: 10.18632/oncotarget.12441

Figure 4. Specific cytotoxicity of TCR gene-transduced CD3+T cells against HLA-A11+ K562 cells as determined by calcein-AM release assay.

Figure 4

Three days after transduction, CD3+T cells transduced with pIRES, TCR Vα13-IRES-Vβ21, and TCR Vα18-IRES-Vβ21 were co-cultured with pEGFP-N3+K562, HLA-A11+ K562 and HLA-A11+ K293 cells at a 40:1 ratio for 4 h. Then, the calcein-AM level in the supernatant was determined. The spontaneous release of calcein-AM from both target and effector cells was subtracted from the measured values, and the final results are expressed as the percentage of specific cytotoxicity. ** , < 0.05. Error bars, S.D.