Table 1. The sequence of primers for PCR.

Primers	Sequence	Function
VA13-F	5′-TCGGCTAGCGGAGCAAGAAGGC AAAGCATC-3′	Sense primer for TCR α13 genes in the 5′ position of IRES containing Nhe I restriction enzyme sites
VA18-F	5′-TCGGCTAGCTGAGCAGGAAACA TGGAGAAGAAT-3′	Sense primer for TCR α18 genes in the 5′ position of IRES containing Nhe I restriction enzyme sites
CA-R	5′-ATTGAATTCGCGAGGGAGCACA GGCTGTCTTAC-3′	Antisense primer for TCR α13 and α18 genes in the 3′ position of IRES containing EcoR I restriction enzyme sites
VB21-F	5′-TAAGCTAGCCTCCCATCCTTCCC TGACCCT-3′	Sense primer for TCR β21 genes in the 5′ position of IRES containing Nhe I restriction enzyme sites
CB-R	5′-GCCGAATTCTCAGCCTCTGGAAT CCTTTCTCTTGAC-3′	Antisense primer for TCR β21 genes in the 3′ position of IRES containing EcoR I restriction enzyme sites
grmb-f	5′- AGATGCAACCAATCCTGCTT-3′	Sense primer for granzyme B
grmb-r	5′-CATGTCCCCCGATGATCT-3′	Antisense primer for granzyme B
perf-f	5′- CCGCTTCTCTATACGGGATTC-3′	Sense primer for perforin
perf-r	5′- GCAGCAGCAGGAGAAGGAT-3′	Antisense primer for perforin
gapdh-f	5′-AGCCACATCGCTCAGACAC-3′	Sense primer for GAPDH
gapdh-r	5′-GCCCAATACGACCAAATCC-3′	Antisense primer for GAPDH