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. 2016 Oct 5;7(51):84286–84298. doi: 10.18632/oncotarget.12483

Figure 4. Radiosensitizing mechanisms of GANT61.

Figure 4

(A) DNA damage response after 72 h treatment with GANT61 (10 μM) prior to/during IR (4 Gy). (B) Cell cycle distribution, (C) Cyclin D1, PARP, cleaved PARP, pAkt and Akt protein expression levels. Samples were pretreated with GANT61 (10 μM) for 72 h prior to IR (4 Gy) and proteins were lysed 24h after IR. Protein expression levels of indicated proteins were also assessed by means of densitometry (relative values indicated below the blots) (D) sub-G1 fraction (E) Annexin V-positive/PI-negative cells. For (A, B), cells were fixed at 1 h, 8 h and 24 h after IR and for (D, E), cells were analysed at 24 h after IR. Means ± SEM of 3 independent experiments. *p < 0.05 vs. control; #p < 0.05 vs. GANT61; $p < 0.05 vs. IR.