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. 2016 Oct 5;7(51):84286–84298. doi: 10.18632/oncotarget.12483

Figure 5. Role of p53 in radiosensitizing effect of GANT61.

Figure 5

(A) p53 and p21 protein expression in 22Rv1 exposed to different treatment modalities. (B) p53 protein expression after GANT61 and/or IR. Samples were pretreated with GANT61 (10 μM) for 72 h prior to IR (4 Gy) and proteins were lysed 24 h after IR in 22Rv1 cells. (C) Clonogenic survival curves of PC3 cells after 72 h treatment with GANT61 (10 μM) prior to/during IR. PC3 cells were transfected with WT p53 48 h prior to IR. Means ± SEM of 3 independent experiments performed in triplicate. *p < 0.05 vs. control. p53 and p21 protein overexpression were validated with western blotting. Protein expression levels of indicated proteins were also assessed by means of densitometry (relative values indicated below the blots).

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