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. 2016 Oct 8;7(51):84299–84313. doi: 10.18632/oncotarget.12520

Figure 6. Dicer decreased the expressions of MMP-2 and VEGFA.

Figure 6

The expressions of MMPs and the inhibitor of MMPs, such as MMP-2, MMP-9, TIMP-2 and TIMP-2 in the 786-O and ACHN cells with different Dicer expression levels were determined by western blot (A). The intensity of the MMP-2 protein bands was analyzed by densitometry, after normalization to the corresponding beta-actin level (B). Gelatin zymography revealed the enzyme activity of MMP-2 in Dicer knockdown groups and control groups (C, D). ELISA assay showed the expression levels of secreted VEGFA (pg/ml) in the conditioned medium collected from the 786-O and ACHN cells with different Dicer expression levels (E). The data are means ± standard deviations from three independent experiments. Data were presented as mean ± SD, *P < 0.05, **P < 0.001; Analysis of Variance.