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. 2004 Nov 29;32(21):6187–6199. doi: 10.1093/nar/gkh958

Figure 2.

Figure 2

(A) Partial resistance to MspI challenge. pUC-cviPIIM was incubated with MspI or ScrFI. MspI does not cleave mCCGG whereas ScrFI cleaves mCCNGG. (B) Identification of methylation site. DNA sequences of PCR products derived from sodium bisulfite-treated pUC-cviPIIM were compared with those from untreated pUC-cviPIIM. The change of cytosine to thymidine corresponds to unmodified cytosine, whereas cytosine in both sodium bisulfite-treated and un-treated plasmids indicates C5-methylcytosine. The recognition sites stated on top of each panel are underlined in red and modified nucleotides are indicated by down arrows.