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(A) The CviPII N-M system contains 2332 nt and two complete ORFs. The cviPIIM gene contains m5C DNA MTase motifs I through VIII as indicated (see also Figure 2). The cviPIINt gene contains two Type II REase active site motifs (see also Figure 2B). (B) Purified recombinant Nt.CviPII was analyzed on a 4–10% SDS–PAGE.
