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. 2004 Nov 29;32(21):6187–6199. doi: 10.1093/nar/gkh958

Figure 5.

Figure 5

Run-off sequencing of Nt.CviPII-cleaved DNA. The sequences were read as reverse-complement by the sequencing primer. Therefore, TGG corresponds to CCA, CGG to CCG, GGG to CCC and AGG to CCT. Triplet sequences in red boxes are CCN sites that are added to the substrate DNA. Those in green boxes are native CCA sequences of pUC19. Arrows under the chromatographs indicate the nt adenine added by the template-independent activity of Taq DNA polymerase used in the sequencing reactions. Arrow heads also indicate the nicking site (complement).