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. 2016 Oct 26;7(51):84416–84427. doi: 10.18632/oncotarget.12936

Figure 4. Effects of 5-aza-dC treatment on cell cycle, apoptosis, senescence, and proliferation in TPC-1 cells.

Figure 4

(A) Flow cytometry confirmed cell cycle arrest at the G1 phase after treatment with 5-aza-dC in TPC-1 cells. (B) AnnexinV staining indicated a significantly greater percentage of TPC-1 cells undergoing apoptosis after treatment with 5-aza-dC. (C) 5-aza-dC significantly increased the number of senescent (β-galactosidase positive) TPC-1 cells (100× magnification, Scale bar = 100 μm). (D) TPC-1 cell growth was inhibited after 5-aza-dC treatment. *p < 0.05 compared untreated TPC-1 cells or TPC-1 cells + PBS using a Student's t test.