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. 2016 Sep 2;7(51):84556–84574. doi: 10.18632/oncotarget.11821

Figure 5. Role of integrins in acquired triapine resistance of SW480/tria cells.

Figure 5

A. and B. Levels of integrin αv, α5, β1, and β5 in plasma membrane-enriched protein fractions (A) and in total protein extracts prepared from SW480 and SW480/tria cells treated as indicated (B) were determined by Western blotting. β-actin was used as loading control. C. SW480 and SW480/tria cells were treated with the indicated concentrations of triapine alone and in combination with cilengitide. After 72 h treatment, cell viability was determined by MTT assay. The values given are means and SD of one representative experiment out of three performed in triplicate. Statistical analysis was performed by two-way ANOVA (***P < 0.001). D and E. SW480 and SW480/tria cells were treated with the indicated concentrations of triapine alone and in combination with specific inhibitory antibodies for integrin α5 (D) or integrin αv (E). After 72 h treatment, cell viability was determined by MTT assay. The values given are means and SD of three independent experiments performed in triplicates. Statistical analysis was performed by two-way ANOVA (*P < 0.05, ***P < 0.001).